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In oxidative stress–oriented in vitro models, such as primary porcine theca cells, a broader dose–response evaluation revealed that EV treatment (30–120 μg/mL for 24 h) reduced reactive oxygen species accumulation, limited apoptosis, and enhanced steroidogenic activity, underscoring the importance of aligning EV dose and duration with the specific stress paradigm and cellular target (Yuan et al., 2022)

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